高压氧暴露下肝癌(H22)超声固化瘤苗

免疫的动物实验研究

李宁1，李启明2

(第三军医大学新桥医院:1.高压氧科;2.肿瘤科，重庆400037)

摘 要 目的 探讨肝癌(H22)超声固化瘤苗在高压氧环境下诱发小鼠的抗肿瘤效应及对免疫功能的影响。方法 将实验小鼠随机分为对照组、常压瘤苗组和高压氧瘤苗组，采用肝癌(H22)超声固化瘤苗对小鼠进行免疫实验，通过癌细胞攻击实验和胸腺细胞自发性3H-TdR掺入量测定,观察其在小鼠体内诱发的抗瘤和胸腺免疫效应.结果 A组的抑瘤率为0、B组和C组的抑瘤率分别为83.33%和91.67%、死亡率分别为16.67%和8.33%、生命延长率分别为75%和87%、6w生存天数39.14±5.6天和51.28±3.7天、肿瘤发生率分别为41.66%和25%，其两组与A组比较有显著差异(P < 0.05)，而B组和C组比较有明显差异(P < 0.05)。免疫B组和C组的腺细胞平均数显著上升,而胸腺细胞3H-TdR掺入量明显下降.结论 免疫小鼠可获得对随后接种同源细胞攻击,有免疫保护作用，并产生免疫记忆; 高压氧曝露的免疫小鼠的抗肿瘤效应和免疫力明显强于另外两组，超声固化瘤苗与高压氧结合能显著地提高激活宿主免疫系统,抑制肿瘤生长和延长生命,有望成为一种新的、安全的、有效和无毒副作用的综合治疗新模式。

关键词 高压氧;超声固化瘤苗;免疫治疗;实验研究

The animal experimentation study on animal liver cancer (H22) of megatemperature ultrasound solidification tumor vaccine on hyperbaric oxygen condition

LI Ning, LI qi-ming, Liao Zhong-li

((1. Hyperbaric Oxygen Therapy department 2.Oncology department, Xinqiao Hospital, the Third Military Medical University , Chongqing 400037 , China;)

Abstract : -ive To investigate the anti-cancer results and immunizing effect of ultrasound solidification tumor vaccine of mouse in hyperbaric oxygen circumstance .Methods The hundreds of mouse randomization into control group, ordinary pressure tumour vaccine group, hyperbaric oxygenation ultrasound solidification tumor vaccine group.The immunizing test was taken on ultrasound solidification tumor vaccine circumstance. Results The total remission rate of tumor in A groups, B groups and C group were 0%, 83.33% and 91.67% respectively. The mortality rate were 16.67% and 8.33% in B groups and C group respectively. The life span prolongation rate were 75%,87% respectively.The mean survival time were 39.14±5.6 day 51.28±3.7 day respectively. The tumorigenesis rate were 41.66% and 25% respectively.The B, C group vs A group the difference is remarkable in statistics (P